Targeted DamID reveals differential binding of mammalian pluripotency factors
Cheetham SW, Gruhn WH, van den Ameele J, Krautz R, Southall TD, Kobayashi T, Surani MA, and Brand AH (2018) Development
Mammalian Targeted DamID (“MaTaDa”) is based on the Targeted DamID (TaDa) technique which was initially developed in Drosophila to achieve cell-type specific genome-wide profiling of chromatin-binding proteins and lncRNAs. To adapt this technology to mammalian cells, the TaDa construct is put under the control of a ubiquitous mammalian promoter (PGK). Cell-type specificity is achieved by inserting a Lox-Stop-Lox cassette, which is removed upon expression or activation of a Cre recombinase.
MaTaDa is an alternative or complementary approach to ChIP-seq, but has many advantages: it is robust and reproducible, requires no antibodies and does not depend on cell sorting to achieve cell-type specificity. It can be completed in 3 days from start to finish and can be performed with fewer than 10,000 cells.
Available reagents (require MTA):
Please contact Andrea Brand firstname.lastname@example.org