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Chromatin DamID

Novel changes in chromatin state during neural development revealed by in vivo cell-type specific profiling
Marshall OJ and Brand AH (2017) Nature Communications

Targeted DamID (”TaDa”) permits genome-wide profiling of DNA- and chromatin-binding proteins in specific cell types. The binding pattern of chromatin-binding proteins with TaDa can be used to generate chromatin maps of specific cell types.

For Chromatin TaDa, we have developed Dam-fusions of the following chromatin-binding proteins for targeted expression:

Brahma - a chromatin remodelling protein associated with H2K27ac, represents TrxG chromatin (reagent information below)

HP1a - an H3K9me3 reader, represents HP1-associated heterochromatin (reagent information below)

Polycomb - an H3K27me3 reader, represents PcG chromatin (reagent information below)

Histone H1 - linker histone, enriched in all repressive chromatin (reagent information below)

RNA Pol II - marks actively transcribed chromatin reagent information here

Available reagents (require MTA):

Please contact Andrea Brand a.brand@gurdon.cam.ac.uk

  • pUAST-LT3-DAM –Brm plasmid plasmid map

  • pUAST-LT3-DAM –Pc plasmid plasmid map

  • pUAST-LT3-DAM –HP1A plasmid plasmid map

  • pUAST-LT3-DAM –H1 plasmid plasmid map

  • UAS-LT3-DAM –Brm Drosophila line

  • UAS-LT3-DAM –Pc Drosophila line

  • UAS-LT3-DAM –HP1a Drosophila line

  • UAS-LT3-DAM –H1 Drosophila line